WAY-316606

Boiling point: 601.1±65.0 °C(Predicted)

Density: 1.50±0.1 g/cm3(Predicted)

Solubility: DMF: 3mg/mL; DMSO: 3mg/mL; DMSO: PBS (pH 7.2) (1:10): 0.1 mg/mL

Form: Powder

Color: White to off-white

1.Cell Experiment

Solubility in DMSO: ≥ 100 mg/mL (222.98 mM; Hygroscopic DMSO significantly affects the solubility of the product, please use freshly opened DMSO)

* "≥" means soluble, but saturation unknown.

Preparing Stock Solutions:

Please choose a suitable solvent to prepare the stock solution based on the product's solubility in different solvents. Once the solution is prepared, aliquot and store it to avoid product degradation caused by repeated freeze-thaw cycles.

Storage conditions and shelf life of the stock solution: -80°C for 2 years; -20°C for 1 year. When stored at -80°C, use within 2 years; when stored at -20°C, use within 1 year.

2.Animal Experiment

Please choose the appropriate dissolution method based on your experimental animals and administration route.

For all of the following dissolution schemes, please first prepare a clear stock solution according to the In Vitro method, and then add solubilizing agents in sequence:

— To ensure the reliability of experimental results, the clear stock solution can be appropriately stored according to storage conditions; for in vivo experiment working solutions, it is recommended to prepare them fresh and use them on the same day.

The percentages shown before the following solvents refer to the volume ratio of that solvent in the final solution you prepare. If precipitation or crystallization occurs during preparation, solubilization can be assisted by heating and/or sonication.

Protocol 1

Please add each solvent in order: 10% DMSO, 90% (20% SBE-β-CD in saline)

Solubility: ≥ 2.5 mg/mL (5.57 mM); clear solution

This protocol can yield a clear solution with ≥ 2.5 mg/mL (saturation unknown).

For 1 mL of working solution, add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD saline solution and mix well.

Dissolve 2 g of SBE-β-CD (sulfobutyl ether β-cyclodextrin) powder in 10 mL of saline, fully dissolving to a clear and transparent solution.

Protocol 2

Please add each solvent in order: 10% DMSO, 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (5.57 mM); clear solution

This method can produce a clear solution with ≥ 2.5 mg/mL (saturation unknown). For animal experiments lasting more than half a month, use this method as appropriate.

For 1 mL of working solution, take 100 μL of 25.0 mg/mL clear DMSO stock solution and add it to 900 μL of corn oil, then mix thoroughly.