Sofpironium Bromide

Storage temp: 4°C, sealed storage, away from moisture

Form: Solid

Color: White to Off-white

1. Cell Experiment

Solubility in DMSO: 100 mg/mL (212.59 mM; ultrasonic treatment required. Hygroscopic DMSO significantly affects the solubility of the product. Please use freshly opened DMSO).

Preparing Stock Solutions：

Please select the appropriate solvent for preparing stock solutions based on the product's solubility in different solvents. Once prepared, aliquot the stock solution and store it to avoid product failure caused by repeated freeze-thaw cycles.

Storage conditions and shelf life: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, use within 6 months; when stored at -20°C, use within 1 month.

2. Animal Experiment

Please select an appropriate dissolution protocol based on your experimental animals and route of administration.

The following protocols require first preparing a clear stock solution using the In Vitro method, followed by sequential addition of co-solvents:

— To ensure experimental reliability, the clear stock solution may be stored appropriately according to storage conditions; for in vivo working solutions, it is recommended to prepare them freshly on the day of use.

The percentages indicated before each solvent refer to its volume ratio in the final solution. If precipitation or crystallization occurs during preparation, dissolution can be assisted by heating and/or sonication.

Protocol 1

Please add each solvent in sequence: 10% DMSO, 40% PEG300, 5% Tween-80, 45% Saline.

Solubility: ≥ 2.5 mg/mL (5.31 mM); Clear solution.

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

For example, to prepare 1 mL of working solution: Add 100 μL of a 25.0 mg/mL clear DMSO stock solution to 400 μL of PEG300 and mix well. Then add 50 μL of Tween-80 to the mixture and mix thoroughly. Finally, add 450 μL of saline to adjust the volume to 1 mL.

Preparation of saline: Dissolve 0.9 g of sodium chloride in ddH₂O and bring the volume to 100 mL to obtain a clear and transparent saline solution.

Protocol 2

Please add each solvent in sequence: 10% DMSO, 90% (20% SBE-β-CD in Saline).

Solubility: ≥ 2.5 mg/mL (5.31 mM); Clear solution.

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

For example, to prepare 1 mL of working solution: Add 100 μL of a 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD in saline and mix well.

To prepare 20% SBE-β-CD in saline: Dissolve 2 g of SBE-β-CD (sulfobutylether β-cyclodextrin) powder in saline and bring the volume to 10 mL. Mix until fully dissolved to obtain a clear and transparent solution.

Protocol 3

Please add each solvent in sequence: 10% DMSO, 90% Corn Oil.

Solubility: ≥ 2.5 mg/mL (5.31 mM); Clear solution.

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). Consider using this protocol for animal experiments lasting longer than half a month.

For example, to prepare 1 mL of working solution: Add 100 μL of a 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix well.