Ensartinib dihydrochloride

Storage temp: 0-4℃ for short term (days to weeks), or -20℃ for long term (months).

Solubility: Chloroform (Slightly, Heated), Methanol (Slightly, Heated)

Form: Solid

Color: White

1. Cell Experiment

Solubility in DMSO : 35.71 mg/mL (56.29 mM; ultrasonic co-solubilization; Hygroscopic DMSO has a significant effect on the solubility of the product, use freshly opened DMSO); H2Solubility in O : 33.33 mg/mL (52.54 mM; Ultrasonic Assisted Lysis)

Preparing Stock Solutions：

Please choose the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; Once prepared into a solution, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and duration of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。 Use within 6 months when stored at -80°C, and within 1 month when stored at -20°C.Note: If you choose water as the stock solution, dilute it to the working solution and filter it with a 0.22 μm membrane before use.

2. Animal Control

Choose the appropriate dissolution protocol based on your experimental animal and mode of administration.

For the following dissolution schemes, please prepare the clarified stock solution according to the in vitro method, and then add the co-solvent in turn:

- In order to ensure the reliability of the experimental results, the clarified stock solution can be properly stored according to the storage conditions; It is recommended that you use the working solution for in vivo experiments and use it on the same day;The percentages shown before the following solvents are the proportion of the volume of that solvent in your final solution; If precipitation and precipitation occur during the preparation process, it can be dissolved by heating and/or ultrasound.

Option 1

Add each solvent in order: 10% DMSO,40% PEG300 ,   5% Tween-80, 45% Saline.

Solubility:≥ 2.08 mg/mL (3.28 mM); Clarifying solution.

A clarified solution of ≥ 2.08 mg/mL (saturation unknown) is obtained for this protocol.

For example, take 1 mL of working solution and add 100 μL of 20.8 mg/mL of clarified DMSO stock solution to 400 μL of PEG300 and mix well. Add 50 μL of Tween-80 to the above system and mix well; Then proceed with 450 μL of normal saline to 1 mL.

Preparation of normal saline: 0.9 g of sodium chloride dissolved in ddH₂O and fixed to 100 mL can obtain a clear and transparent normal saline solution.

Option 2

add each solvent in order:10% DMSO,90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.08 mg/mL (3.28 mM); Clarifying solution

A clarified solution of ≥ 2.08 mg/mL (saturation unknown) is obtained for this protocol.

For example, 100 μL of 20.8 mg/mL of clarified DMSO stock solution is added to 900 μL of 20% SBE-β-CD saline in water and mixed well.

Option 3

add each solvent in order:10% DMSO,90% Corn Oil

Solubility: ≥ 2.08 mg/mL (3.28 mM); Clarifying solution

A clarified solution of ≥ 2.08 mg/mL (saturation unknown) is available for use in animal experiments with an experimental period of more than half a month, as appropriate.

For example, 100 μL of 20.8 mg/mL of clarified DMSO stock solution was added to 900 μL of corn oil and mixed well.