Enmetazobactam

Storage temp: Store at -20°C, protect from light, stored under nitrogen

Solubility: DMSO: 113.3(Max Conc. mg/mL); 360.46(Max Conc. mM)

Form: Solid

Color: White to Off-white

1. Cell Experiment​​

​​DMSO:​​ ≥ 113.3 mg/mL (360.46 mM)

*"≥" indicates soluble, but the saturation solubility is unknown.

Preparing Stock Solutions：

​​

Please prepare the stock solution in the appropriate solvent based on the product's solubility in different solvents. Once dissolved, aliquot the solution to avoid product inactivation caused by repeated freeze-thaw cycles.

​​Storage method and shelf life of the stock solution:​​ -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use within 6 months; when stored at -20°C, please use within 1 month.

​​2. Animal Experiment​​

Please select the appropriate dissolution protocol based on your experimental animals and administration method. For the following protocols, first prepare a clear stock solution in vitro, then add co-solvents in sequence:

To ensure reliable experimental results, the clear stock solution can be stored appropriately according to the storage conditions. For in vivo experiments, it is recommended to prepare the working solution fresh and use it on the same day.

The percentages shown before the solvents refer to the volume percentage of each solvent in the final solution. If precipitation occurs during preparation, heating and/or sonication can be used to aid dissolution.

​​Protocol 1​​

Add each solvent in the following order: 10% DMSO, 40% PEG300, 5% Tween-80, 45% Saline

​​Solubility:​​ ≥ 2.08 mg/mL (6.62 mM); Clear solution

This protocol yields a clear solution with a concentration of ≥ 2.08 mg/mL (6.62 mM, saturation unknown).

​​Preparation example for a 1 mL working solution:​​

Take 100 μL of the 20.8 mg/mL clear DMSO stock solution and add it to 400 μL of PEG300, mix well. Then add 50 μL of Tween-80 to the mixture and mix thoroughly. Finally, add 450 μL of normal saline to bring the volume to 1 mL.

​​Protocol 2​​

Add each solvent in the following order: 10% DMSO, 90% (20% SBE-β-CD in Saline)

​​Solubility:​​ ≥ 2.08 mg/mL (6.62 mM); Clear solution

This protocol yields a clear solution with a concentration of ≥ 2.08 mg/mL (6.62 mM, saturation unknown).

​​Preparation example for a 1 mL working solution:​​

Take 100 μL of the 20.8 mg/mL clear DMSO stock solution and add it to 900 μL of a 20% SBE-β-CD solution in saline, mix well.

​​Protocol 3​​

Add each solvent in the following order: 10% DMSO, 90% Corn Oil

​​Solubility:​​ ≥ 2.08 mg/mL (6.62 mM); Clear solution

This protocol yields a clear solution with a concentration of ≥ 2.08 mg/mL (6.62 mM, saturation unknown). ​​This protocol is not suitable for experiments lasting longer than half a month.​​

​​Preparation example for a 1 mL working solution:​​

Take 100 μL of the 20.8 mg/mL clear DMSO stock solution and add it to 900 μL of corn oil, mix well.

Enmetazobactam is an antibacterial agent that is active against most gram-positive and gram-negative bacteria.

Extended-spectrum beta-lactamases (ESBLs) are a group of bacterial serine beta-lactamases that hydrolyze third-generation cephalosporins (3GC), leading to the development of 3GC-resistant bacteria. When used in combination with cefepime, enmetazobactam protects cefepime from degradation by ESBLs and prevents antibiotic resistance.