LGD-3303

Boiling point: 492.0±45.0°C(Predicted)

Density: 1.43±0.1 g/cm3(Predicted)

Storage temp.: Store at -20°C

Solubility: Chloroform (Very Slightly), DMSO (Slightly), Methanol (Slightly)

Form: Solid

Color: Pale Yellow to Light Yellow

1.Cell Experiment

Solubility in DMSO: 10.42 mg/mL (30.40 mM; sonication-assisted dissolution (<60°C); the solubility of the product is significantly affected by hygroscopic DMSO, please use freshly opened DMSO)

Preparing Stock Solutions:

Please choose a suitable solvent to prepare the stock solution based on the product's solubility in different solvents. Once the solution is prepared, aliquot and store it to avoid product degradation caused by repeated freeze-thaw cycles.

Storage conditions and duration for the stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, use within 6 months; when stored at -20°C, use within 1 month.

2.Animal Experiment

Please choose the appropriate dissolution method based on your experimental animals and administration route.

For the following dissolution schemes, please first prepare a clear stock solution according to the In Vitro method, then add solubilizers in sequence:

—— To ensure the reliability of the experimental results, the clear stock solution can be appropriately stored based on storage conditions; for in vivo experimental working solutions, it is recommended to prepare them fresh and use them on the same day;  

The percentages shown before each solvent below refer to the volume proportion of that solvent in the final solution you are preparing; if precipitation or crystallization occurs during preparation, solubilization can be assisted by heating and/or ultrasonic treatment.

Protocol 1

Please add each solvent in sequence: 10% DMSO, 90% (20% SBE-β-CD in saline).  

Solubility: 1.67 mg/mL (4.87 mM); suspension; ultrasound-assisted solubilization.  

This method can produce a uniform suspension at 1.67 mg/mL, which can be used for oral and intraperitoneal administration.  

For example, to make 1 mL of working solution, take 100 μL of a 16.7 mg/mL clear DMSO stock solution and add it to 900 μL of 20% SBE-β-CD in saline, then mix thoroughly.  

Dissolve 2 g of SBE-β-CD (sulfobutyl ether β-cyclodextrin) powder in 10 mL of saline, completely dissolving to a clear, transparent solution.

Protocol 2

Please add each solvent in sequence: 10% DMSO, 40% PEG300, 5% Tween-80, 45% Saline.

Solubility: 1.04 mg/mL (3.03 mM); suspension; ultrasound-assisted solubilization.

This method can produce a uniform suspension at 1.04 mg/mL, which can be used for oral or intraperitoneal administration.

For example, to prepare 1 mL of working solution, take 100 μL of 10.4 mg/mL clear DMSO stock solution and add it to 400 μL of PEG300, mix well; then add 50 μL of Tween-80 to the above mixture and mix thoroughly; finally, add 450 μL of saline to bring the total volume to 1 mL.

Preparation of saline: Dissolve 0.9 g of sodium chloride in ddH₂O and bring the volume up to 100 mL to obtain a clear, transparent saline solution.

Protocol 3

Please add each solvent in order: 10% DMSO, 90% Corn Oil

Solubility: ≥ 1.04 mg/mL (3.03 mM); clear solution

This method can achieve a clear solution with ≥ 1.04 mg/mL (saturation unknown). For animal experiments lasting more than half a month, use this method as appropriate.

For 1 mL of working solution, take 100 μL of 10.4 mg/mL clear DMSO stock solution and add it to 900 μL of corn oil, then mix thoroughly.